Improving the shelf life of rose, sunflower and lisianthus cut flowers using sodium nitroprusside

Number of pages: 54 File Format: word File Code: 32491
Year: 2013 University Degree: Master's degree Category: Agricultural Engineering
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  • Summary of Improving the shelf life of rose, sunflower and lisianthus cut flowers using sodium nitroprusside

    Master's thesis in the field of agricultural engineering - horticultural sciences

    Treatment: ornamental plants

    Abstract

    In order to investigate the effect of sodium nitroprusside on the flowering life of cut flowers of roses, sunflowers and lisianthus, a study in the form of a factorial experiment based on a completely randomized design with Two factors of sodium nitroprusside at four levels (0, 20, 40 and 60 ?M) were performed in 12 treatments, 3 replications and 36 plots with 180 flower branches. In this experiment, traits such as flower diameter (flower diameter reduction index), floret count, dry matter percentage, brix reduction, flower life, water absorption, petal protein content, bacteria count in flower solution and stem, leaf chlorophyll content and ethylene content were evaluated. The longest flowering time was obtained in roses with a concentration of 20 micromolar sodium nitroprusside and sunflower with a concentration of 60 micromolar sodium nitroprusside. Treatment with sodium nitroprusside increased the amount of water absorption, Brix degree and percentage of dry matter compared to the control treatment. The current research shows that by using appropriate concentrations of sodium nitroprusside, the life after harvesting of cut flowers of rose, sunflower and lisianthus can be increased. style="direction: rtl;">History, botany and distribution of roses

    The antiquity of roses goes back to ancient times. Rose fossils are from 35 to 70 million years ago. In ancient Greek and Roman civilizations and in mythology and literature, roses are mentioned as a symbol of love, beauty and happiness. Wild roses have existed since prehistoric times, and about 150 species of them have grown in Europe, Asia, and the Middle East, and were later cultivated and bred by humans (Godin, 2001). In the 12th century AD, this flower was introduced to Europe, and because of its bright red color, it was called a rose. For the first time, Michel Adanson, a French naturalist, chose the name rose for the rose family in 1763 to 1963 (Haywood, 2007).

    Rose with the scientific name Rosa hybrida belongs to the rose family, and in terms of growth, bush shape and flower production, it is classified as miniature, patio, hybrid tea, floribanda, shrub and high roses. The process is divided. The plants of this family are herbaceous, tree or shrub. Rose has specific, regular, bisexual and sometimes unisexual flowers, in which the petals are free. The leaves of the alternate rose and its stems have blades, although the original and old varieties had very thin stems. There are 5 sepals and 15 or more stamens in this flower and the seeds are albumenless. The ovary is upper or lower, which depends on its sex (Wisman and Ritz, 2007). The current development is sustainable economic growth. Flowers and ornamental plants are among the products that have the ability to earn currency and can be included as one of the major non-oil products in the export of goods (Idrisi, 1388). England, America, Switzerland, Italy, Austria, Denmark, Sweden, Belgium, Luxembourg and Japan are among the important flower importers in the world (Anonymous, 2005).

    Iran ranks 17th in terms of flower production and ranks 107th among 117 countries in terms of flower export, which is not a good place in the list of exporters. In the field of cultivation, rose ranks second after gladiolus in Iran. The area under the cultivation of cut flowers of greenhouse and outdoor roses is about 400 hectares, and Isfahan province is the first in the production of roses, especially Dutch roses, and Tehran and Central provinces are in the next ranks (Kyani).The cultivated area of ??cut flowers of greenhouse and outdoor roses is about 400 hectares, and Isfahan province has the first place in the production of roses, especially Dutch roses, and Tehran and Central provinces are in the next ranks (Kiani and Kafi, 2004). From the Helianthus genus in the Asteraceae family, native to North America, it has sun-like inflorescences with a diameter of about 15 cm. This flower has a strong and hairy stem and rough and large leaves. Fertilization is often heterozygous.

    Sunflower is classified as a day-indifferent plant because it flowers over a wide range of day lengths. This flower grows well in soils whose texture changes from sandy to clay.

    1-4- Economic importance of sunflower

    Sunflower under the ship has between 150-100 hectares in greenhouse and open space, located in Tehran, Central and Isfahan provinces.

    1-5- Botany of Lisianthus flower

    Lisianthus, with the scientific name Eustoma qrandiflorum (Raf.) Shin, belongs to the Gentianaceae family, has interesting flowers and long stems (Lugasi et al., 2010), native to the United States of America from Wyoming, South Dakota, Nebraska, Colorado, Kansas, Oklahoma, Texas and especially northern Mexico (Shiner, 1957; Wood and Weaver, 1982). Lisianthus is one of the ornamental plants that was produced as an ornamental flower in Japan more than 60 years ago (Okawa et al., 1991), but since the 1980s it has been introduced into the flower trade as a cut flower (Holloway and Kufrank, 1984). This species is a self-fertile diploid (Shamir et al., 1999) and propagated by seeds (Islam et al., 2005).

    Lisianthus flowers are divided into four groups in terms of the shape of the calyx: funnel, cup, bell, and bowl-shaped (Kawabata et al., 2009). The flowers are sparse and abundant in small, medium and large sizes and are formed singly or in multiples on the leafy stem (Qassimi and Kafi, 1389). has Currently, lisianthus is available in different colors and shapes and is cultivated as a cut flower in a wide area of ??the world. In England, about 10 hectares of gardens have been cultivated with lisianthus. America, Holland, Occupied Palestine, Kenya, Tanzania and Japan are important producers of this plant (Okawa and Sazaki, 1999).

    Lisianthus cut flower production in 2088 was about 56928000 branches in an area equal to 26.5 hectares. Therefore, about 2.8% of the country's cut flower production in 1988 was related to lisianthus.

    In 2001, the average price of lisianthus in California was 10 dollars for each package containing 10 stems. In Iran, each package is sold for 1000-4000 tomans depending on the time of release.

    1-7- Statement of the problem

    In flowers, most of the metabolic processes involved in aging are irreversible, and this is mostly related to the production of ethylene, which reaches its maximum amount during the aging stage of flowers (Selgi et al., 2009).

    Therefore, treatment with suitable protective solutions is recommended to prolong the life of cut flowers. (Manshizadeh et al., 2013).

    Nitric oxide gas is effective in increasing the life of flowers after harvesting. This gas regulates the activity of internal ethylene (Hashimabadi, 2013).

    1-8- The purpose of the experiment

    1- The effect of sodium nitroprusside in increasing the life of the flowers and the quality of cut flowers of roses, sunflowers and lisianthus.

    2- Introduction of the best concentration of sodium nitroprusside in increasing the life Rose, sunflower and lisianthus cut flowers.

    3- Investigating the antimicrobial properties of sodium nitroprusside.

  • Contents & References of Improving the shelf life of rose, sunflower and lisianthus cut flowers using sodium nitroprusside

    List:

    Abstract. 1

    Chapter One: Introduction

    1-1- History, botany and distribution of roses. 3

    1-2- The economic importance of roses. 4

    1-3-Botany and ecological distribution of sunflower. 4

    1-4- The economic importance of sunflower. 5

    1-5- Botany of lisianthus flower. 5

    1-6- The economic importance of lisianthus flower. 5

    1-7- statement of the problem. 6

    1-8- The purpose of the test. 6

    Chapter Two: Review of sources

    2-1- Aging. 8

    2-2- Ethylene and its role in the aging of cut flowers. 8

    2-2-1- Effective factors in ethylene production. 9

    2-2-2- ethylene biosynthesis. 9

    2-3- Treatment with anti-ethylene substances. 10. 2-3-1- sodium nitroprusside. .10

    2-4-vascular obstruction. 11

    Chapter Three: Materials and Methods

    3-1- Plant materials. 13

    3-2- How to prepare flowers and perform sodium nitroprusside treatment. 13

    3-3- implementing the experimental plan and introducing the treatments. 13

    3-4- Quantitative and qualitative traits to be measured. 15

    3-4-1- Omar Goljaei. 15

    3-4-2- solution absorption. 15

    3-4-3 percent of dry matter. 15

    3-4-4- counting bacteria. 15

    3-4-4-1- Preparation of cultivation medium. 16

    3-4-4-2- counting bacteria in pulse solution. 16

    3-4-4-3- counting bacteria in the stem. 16

    3-4-5- The process of flower opening and flower diameter reduction. 17

    3-4-6- Brix reduction (the percentage of sucrose in the flower stem). 18

    3-4-7- Chlorophyll content. 19

    3-4-8- Ethylene measurement. 19

    3-4-9- Petal protein content. 20

    3-5- Data analysis. 20

    Chapter four: Results and discussion

    4-1- Omar Goljaei. .22

    4-2- Brix degree. .25

    4-3- Stem bacteria. 27

    4-4- Soluble bacteria. 29

    4-5- The opening of flowers and florets. 31

    4-6 percent of dry matter. 33

    4-7- Solution absorption. 35

    4-8- Ethylene. 37

    4-9- Chlorophyll b.39

    4-10- Chlorophyll a.41

    4-11- Petal protein.43

    4-12- Discussion. 46

    General conclusion. .48

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Improving the shelf life of rose, sunflower and lisianthus cut flowers using sodium nitroprusside