Determining the economic damage threshold of golden nematode Globodera rostochiensis on potato cultivars

Number of pages: 93 File Format: word File Code: 32489
Year: 2013 University Degree: Master's degree Category: Agricultural Engineering
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    Dissertation for Master's Degree in Agricultural Engineering

    Plant Pathology Orientation

    Abstract

    The nematode Globodera rostochiensis had not been reported from Iran before 1387. The high contamination of the nematode in the potato fields of Hamedan province is a serious threat to production Potato, which is the economic product of this region.

    Considering the seriousness of Globodera rostochiensis damage to Iran, so far not much information has been collected about determining the economic damage threshold of the nematode in cultivars cultivated in Hamadan province. Therefore, this research was investigated for the first time in Iran on two potato varieties, Marfona and Sante. Contaminated soil samples were collected from Hamedan province and after phenocrysts cysts were isolated to prepare the nematode population. This research was carried out in seven treatments with three repetitions. The first control treatment and then the treatments included 2, 4, 6, 8, 16, 32 and 64 seeds per gram of soil.

    After cultivating the two cultivars in four kilo pots containing sterile soil after rooting, the populations prepared around the roots were inoculated and harvested after three months of the treatments. and the results were analyzed.

    According to the statistical investigations on growth factors and nematodes that were carried out with Mstatc software, it was concluded that Sante variety is resistant to Globodera rostochiensis, and in the case of Marfona variety, according to the hatching rate of nematode factors, the economic loss level of four eggs per gram of soil was obtained.

    Key words: Globodera rostochiensis, golden cyst nematode, resistant potato varieties, Marfona variety, Sante variety, level of economic loss of golden nematode

    1 History of potato

    Potato from the Solanaceae family is one of the best agricultural products that is very important as a valuable food and industrial material in most parts of the world.

    According to archeological studies, from seven to eight thousand years ago, farmers in Peru have cultivated wild types of potatoes. Based on this, the researchers have declared the origin of this plant in the Andes region of Peru. According to the researchers, the Inca Indians discovered it for the first time in 2000 BC, and in 1537, the Spanish soldiers became aware of its cultivation by the Inca Indians, who called it Paya. This product then entered Spain and from there to Italy and other countries. It spread to Central Europe and gradually became a staple food product in Europe, especially in Germany, Russia and Ireland (Hasanpanah; Nikshad and Hosni, 1378).

    Some others believe that potatoes were first sent from Ireland to England. An English lord named

    "Servalter Raleigh" cultivated it in his farm near "Kirk" and later it went to Germany, Austria and Russia (Omidwar, 1348).

    In the early 17th century, potatoes were offered in the palaces of the kings and then in the palaces of European nobles as an expensive food item. The 19th planting of potatoes was used as a raw material for consumption in alcohol and starch factories. Anyway, its main origin is in South America, where it still grows wild in some places, and its wild varieties that show resistance to the disease are used to obtain new domestic varieties.

    In recent years, potatoes were imported to many countries with hot and dry climates and in areas such as North Africa, the plains of India, Bangladesh, Pakistan, Central Africa, China, Argentina, Uruguay and The plains of the coast of Peru have been developed.

    1-2 Botanical characteristics of potato

    Potato with the scientific name Solanum tubersoum botanically belongs to the Solanaceae family, in which there are other important plants such as tomatoes, eggplants, and peppers. The species of this genus are abundant in tropical regions and their abundance gradually decreases towards temperate and cold regions (Kaherman, 1373). According to the studies conducted, different potato species are distributed from Chile to the south of the United States and from sea level to an altitude of 4800 meters.

               The origin and the main area of ??distribution of this genus is the western regions of South America, i.e. the countries of Peru, Chile, Bolivia, Ecuador, where about 40 species of this family grow there. Ten species of it are distributed in Europe, 15 species in Asia, and finally 10 species in Africa, and among these, 12 species of this genus are also scattered in different regions of Iran. and food should be considered as one of the important genera of plants (Falahi, 1376).

    In potato, the number of chromosomes in the genus Solanum is twelve. The mini apple is a herbaceous and perennial plant, but it is cultivated as an annual plant in agriculture. This plant usually reproduces through the seed tuber. From the buds on the seed tuber, branches and organs Aerial roots are created. Roots are formed from primordia on buds. On these branches and aerial organs, stems and leaves, stolons, roots and inflorescences, as well as the next generation of tubers, are formed. style="direction: rtl;">General morphology:

    The morphology of the upper and lower parts of the soil surface in potatoes depends on the physiological age of the cultivated seed tubers, and in this way, the growth pattern of each single stem according to its initial strength, the number of leaves formed on each stem, the time of stolon formation, the time of growth and branching of stolons, the beginning of tuber formation and flowering, the duration of formation and growth tubers, the beginning of senescence and the length of growth and growth period of the plant are affected.

    Stem system:

    Since each eye on the potato seed tuber can produce more than one stem. And considering that on each seed tuber, many buds have the ability to produce stems, it is natural that a number of stems are formed from each tuber. Therefore, in the field, a set of stems branch out from a potato seed tuber in the form of a cluster. In the early stages of growth, these stems provide their food resources from the seed tuber, but in a short distance after the sprouting of each stem, they reach the stage of self-sufficiency and independence, and at the same time, they compete with other adjacent stems for light, water, and food. Usually two types of stems. One with definite growth and the other with indeterminate growth are observed in potato cultivars. In cultivars with definite growth, the height of the stems is short and each stem has a short life span. The yield potential in indeterminate cultivars is usually higher than that of cultivars with definite growth, but to realize it, the length of the growing season must be long so that the plant can reach its yield potential. The stems in the underground part of the plant are usually round and plump, but in the aerial parts of the plant in the middle of the node, they may be hollow. In addition, in the aerial parts of the plant, the stems are angular and angular. and can be seen in red to purple colors.

    Leaves:

    The potato plant has one compound leaf in each node of the stem. The initial leaves that appear at the beginning of potato growth are small in size, but the subsequent leaves are compound with 3-4 oval leaflets each. Potatoes have long days. And high temperatures increase the number of leaves in the secondary stems compared to the main stem.

  • Contents & References of Determining the economic damage threshold of golden nematode Globodera rostochiensis on potato cultivars

    List:

    Abstract 1

    Chapter One: Introduction

    1-1- History of potato 2

    2-1-Botanical characteristics of potato 4

    1-2-1- Figures Marfona 8

    1-2-2-Sante number 8

    1-3- Importance and nutritional value of potato 9

    1-4- Potato diseases 10

    1-5- Necessity of apple cyst nematode management 11

    6-1-Research objectives 11

    1-2-1- Research hypotheses 12

    Chapter Two: review and review of sources

    2-1-Plant parasite nematodes 14

    2-2- Importance of plant nematodes 16

    2-3 Potato golden nematode cyst 18

    2-3-1- History of emergence and geographical distribution of potato cyst nematode 18

    2-3-2-Globodera position in taxonomy Nematodes 18 2-4-Nematode characteristics and pathogenicity of Globodera rostochiensis 22 2-4-1-Morphological and morphometric characteristics 22 2-4-2 Biology and histology of the disease 24 2 5- Economic damage threshold of nematodes 26 2-5-1 Economic damage of Globodera rostochiensis 28 2-6 Nematode control solutions 30 2-6-1 Agricultural measures 30

    2-6-2-Sanitary measures 30

    2-6-3-Biological control 31

    2-6-4-Chemical control 31

    2-6-5-Integrated management 32 Chapter Three: Materials and Methods 3-1 - Collection of contaminated soil 34 3-2 Extraction of cysts by Fenwick method 34 3-3 How to prepare Cone top and identify cysts 35 3-3-1 Preparation of glycerin gel 36 3-4 - Determining the population of nematode eggs and larvae in the soil 36 3-5 - Preparation of soilHistory of potato 2-1-Botanical characteristics of potato 4-1-2-1- Marfona variety 8-2-2-Sante number 8

    1-3- Importance and nutritional value of apple 9

    1-4- Potato diseases 10

    5-1- Necessity of potato cyst nematode management 11

    1-6 - Research objectives 11

    1-2-1- Hypotheses Research 12 Chapter Two: Review and review of sources 2-1-Plant parasitic nematodes 14 2-2 Importance of plant nematodes 16 2-3 Potato golden nematode 18

    2-3-1- The history of the emergence and geographic distribution of the potato cyst nematode 18

    2-3-2-Globodera's position in the classification of nematodes 18

    2-4-Nematode characteristics and pathogenicity of Globodera rostochiensis 22

    2-4-1-Morphological characteristics and Morphometric 22 2-4-2- Biology and histology of the disease 24 2-5- Economic damage threshold of nematodes 26 2-5-1- Economic damage of Globodera rostochiensis 28 2-6- Solutions Nematode control 30

    2-6-1- Agricultural measures 30

    2-6-2- Sanitary measures 30

    2-6-3- Biological control 31

    2 6-4-Chemical control 31 2-6-5 Integrated management 32 Chapter 3: Materials and methods 3-1 - Collection of contaminated soil 34 3-2 - Extraction of cysts by method Fenwick 34 3-3 - How to prepare Cone top and identify cysts 35 3-3-1 - Preparation of glycerin gel 36 3-4 - Determining the population of nematode eggs and larvae in the soil 36 3-5 - Preparation of soil Sterile 37 3-6 - Preparation of seeds and preparation for planting 38 3-7 - Inoculation of eggs and larvae 39 3-8 - Review of treatments 40 Chapter 4: Results and Discussion 4-1- Detection of golden potato nematode 40 4-2 Evaluation of changes in growth factors and nematodes on Marfona and Sante potato cultivars 41 4-2-1 Review of the average comparison table of treatment combinations 44 4-2 2- Average comparison table Treatments 51 Chapter Five: Conclusion 5-1 – Conclusion 71 5-2 – Suggestions 74 Sources 75

    Appendix 83

    English abstract 96

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    5) Hassan Panah, D. Nikshad, Khalil. Hosni, M. 1387. Ardabil, Hafez Andisheh. 193 p.

    6) Hassanzadeh, Nader 1371. Biocontrol of soil-borne pathogenic agents of plants, Publications of the Research Institute of Pests and Plant Diseases, 169 p.

    7) Damadzadeh, M. 1389. Nematology in agriculture Andisheh Gostar Publications, 208 p. Potato Diseases, translated by Abazar Rajabi, University Publishing Center, Tehran, 453 p.

    9) Drapkin, Victor. 1375. An Introduction to Plant Nematodology, translated by Behrouz Jafarpour and Ismat Mahdikhani Moghadam, Ferdowsi University Press, Mashhad, 360 p.

    10) Rich 1370. Potato diseases, translated by Behrouz Jafarpour, University of Mashhad, 283 pages.

    11) Kazemi, M. Hasanabadi, H. Tavakoli, 1390 Management of potato production. Tehran, Agricultural Education and Extension Publications. 160 p. 12) Fallahi, M. 1376. Potato Knowledge and Technology (Translation). Yarthava Publications. Tehran. 13) Kahraman, Ahmad. 1373. Chromophytes of Iran, Plant Systematics, Volume 3, Tehran University Press, First Edition.

    14) Gitti, M. Tanha Mofi, 1387. Report on the presence of potato quarantine nematode in Hamadan province. The 18th Iran Botanical Congress. Hamedan province agricultural and natural resources research center and the country's agricultural research institute. Prior, 426 p.

    16) Nasr Esfahani, M. Ahmadi, 1388. Principles of Nematodology, Gholami Publications, 334 p.

    17) Agrios, G.

Determining the economic damage threshold of golden nematode Globodera rostochiensis on potato cultivars