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  3. Extraction and determination of Proscillaridin in Ansal onion native to Iran

Extraction and determination of Proscillaridin in Ansal onion native to Iran

Number of pages: 74 File Format: word File Code: 32394
Year: Not Specified University Degree: Not Specified Category: Agricultural Engineering
Tags/Keywords: Ansal onion b - Drimia maritima L - HPLC - Medicinal plants - Proscillaridin - traditional medicine - Use cases
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  • Summary of Extraction and determination of Proscillaridin in Ansal onion native to Iran

    Persian summary

    Ansul onion, with the scientific name Drimia maritia, is one of the important medicinal plants that has been used in traditional medicine to treat various diseases, including swelling, respiratory disorders, jaundice, and epilepsy.

    Bufadinolides have been identified as the main compounds of this plant. Bufadinolide compound of this plant is Proscillaridin, which has many pharmacological properties. Among the therapeutic effects of prosilaridin, we can mention the following: effectiveness against congestive heart failure, antitumor activity, immunosuppression and analgesic activities. Due to the pharmacological importance of Proscillaridin, in this research, this compound was extracted from Drimia maritia plant in Iran and quantified by valid HPLC method. In order to fully extract Proscillaridin and separate it from other plant compounds, methanol was used as the best extraction solvent and sonification method was used as the best extraction method. Analytical was injected. Proscillaridin was isolated by using a methanol-water gradient system and using a column (6 mm 5µm/4x250ACE C18) and a solvent flow rate equal to 1 ml/min.  The analytical method used to determine the amount of Proscillaridin, which is the most important compound of the plant, was validated. Validation of the method was done using the parameters of selectivity, linearity (r=0.9982), accuracy between days (4.8%), accuracy in one day (0.12%) and accuracy (93.7%). The limit of detection and the limit of quantification have been determined as 0.6 and 1.8 micrograms/ml, respectively. The used method has acceptable precision and accuracy, so it can be used as a UV-HPLC method to determine the amount of Proscillaridin. rtl;">1-1. Necessity and importance of the subject

    Ansal plant with the scientific name Drimia maritima L. is one of the native plants of Iran whose use in native medicine has been common since the ancient civilizations of Iran and Greece. Among the uses of this plant in traditional medicine, expectorant, anti-epileptic and anti-jaundice effects can be mentioned. The main compounds of this plant are cardiac glycosides, which in addition to the known effects on the heart, the anti-cancer effects of these compounds have also been reported in several studies. In this regard, the effects of Ansal onion have been proven in breast cancer and lymphoma, and among the many bufadinolide compounds of this plant, Proscillaridin is known as an effective substance.  Despite the research that has been done so far on this valuable plant, no information has been published in the databases about Ansal onion native to Iran. Due to the increasing spread of traditional medicine and the wide use of this plant in the products available in traditional medicine clinics, it is necessary to determine the amount of the effective substance in order to standardize the products that are prepared from Ansul onion in traditional medicine treatment centers and provided to patients. Therefore, determining the amount of the active ingredient Proscillaridin by high-performance liquid chromatography (HPLC) has been investigated as the subject of this research project.

    1-2. Statement of the problem

    According to the side effects that may occur due to the use of chemical drugs, nowadays the tendency to use herbal drugs that have less side effects has increased. Our country, Iran, has a rich history in the field of traditional medicine. One of the medicinal plants used in traditional Iranian medicine is Ansal onion with the scientific name Drimia maritima L. (1). This plant grows in Fars and Khuzestan provinces (2). Among the uses of Ansal in traditional medicine, we can mention diseases such as epilepsy, shortness of breath, cough, jaundice, and also locally for joint pain and gout (3, 4). The effects that have been proven during many studies of Ansal onion include: cardiac effects similar to digitalis, heart stimulant, antibacterial, diuretic, effect on gout and rheumatism, as well as effect on lymphoma and lung cancer (7, 6, 5).The most important compounds in this plant are bufadinolides from the cardiac glycosides category, among which the largest amount is related to Scillaren A, Scilliroside, Proscillaridin A (6, 8). Cardiac glycosides cause positive inotropic effects on the heart by inhibiting the Na+/K+- ATPase pump (9). These compounds have a low therapeutic index, and even in therapeutic doses, they may cause side effects such as increased heart muscle excitability, irregular heart rhythm, vomiting, diarrhea, loss of appetite, and headache. (6, 9) In order to take advantage of the therapeutic properties of Ansal plant and control its side effects, by determining the amount of effective compounds, the most important of which is Proscillaridin, a suitable method can be provided for the standardization of pharmaceutical products prepared from Ansal. Ahedf

    1. Extraction and determination of Proscillaridin amount in Ansal onion native to Iran

    2. Standardization of products prepared from Ansal onion using determination of Proscillaridin amount by HPLC

    3.

  • Contents & References of Extraction and determination of Proscillaridin in Ansal onion native to Iran

    List:

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    Source:

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Extraction and determination of Proscillaridin in Ansal onion native to Iran
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