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  3. Determining the effect of low power Ga-Al-As laser on the proliferation and function of human periodontal fibroblasts (cell line HFG3-P1 53) in an in vitro environment.

Determining the effect of low power Ga-Al-As laser on the proliferation and function of human periodontal fibroblasts (cell line HFG3-P1 53) in an in vitro environment.

Number of pages: 163 File Format: word File Code: 32009
Year: 2012 University Degree: Master's degree Category: Medical Sciences
Tags/Keywords: Collagen - Dentistry - Fibroblast - Fibroblast cell activity - Fibroblast proliferation - HFG3 - Low power laser - Low power laser - marker genes - Periodontium
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  • Summary of Determining the effect of low power Ga-Al-As laser on the proliferation and function of human periodontal fibroblasts (cell line HFG3-P1 53) in an in vitro environment.

    Dissertation to receive the degree of Doctor of General Dentistry

    Persian abstract

    Introduction: Restoration of lost periodontium tissue is the ultimate goal of ideal periodontal treatment. Proliferation of fibroblasts along with regeneration of periodontal tissue is an ideal therapeutic result. The use of low power laser has been one of the proposed methods to accelerate the proliferation of fibroblasts and promote the healing process. For about 50 years, low-power lasers have been used in various fields of dentistry. This study was conducted with the aim of investigating the effect of low-power gallium-aluminum-arsenide laser on the proliferation and function of human periodontium fibroblasts (HFG3-P153 cell line) in an in vitro environment. Materials and methods: In this study, a low-power Ga-Al-As laser device with a wavelength of 810 nm and a maximum power of 50 mW was used. After the cultivation of gingival fibroblasts, the cells were divided into two test and control groups. In the test group, the cells were irradiated for three consecutive days at the rate of 4 J/cm2 each time. From the cells of both the intervention and control groups, at 24, 48, and 72 hours after radiation, MTT test was performed to check cell proliferation and 72 hours after radiation, RT-PCR was performed to check the expression of genes indicating fibroblast cell activity.

    Results: The results of MTT test showed that the test and control groups did not differ significantly from each other at 0 and 24 hours after radiation, but in hours 48 and 72, this difference was significant. The RT-PCR test also determined that collagen gene expression in the intervention group increased 4.7 times compared to the control group.

    Conclusion: The use of low-power Ga-Al-As laser increases the proliferation of fibroblasts and promotes collagen production. Considering the role of fibroblasts in periodontal regeneration and pocket removal, the use of low-power laser in the clinic can increase the efficiency of periodontal treatments. rtl;"> 

     

     

    Introduction

    tissue repair includes the extensive activity of some cells such as epithelial cells, endothelial cells and most importantly fibroblasts that play a key role in this process. Fibroblasts secrete several growth factors during epithelization (1) and actively participate in the formation of granulation tissue and the synthesis of complex extracellular matrix after epithelization. The restoration of a tissue involves the occurrence of a series of consecutive events. As a result of the created wound, a hematoma is formed. Platelets are the first cell components that migrate to the site and start the wound healing process by releasing growth factors. Various chemicals are released by platelets and monocytes, which causes fibroblasts to be absorbed into the hematoma. When fibroblasts migrate to the wound site, they begin to multiply and synthesize collagen. Proliferation of fibroblasts, epithelial and endothelial cells mainly depends on growth factors and collagen deposition (2). The cross-linking of collagen fibers and their accumulation increase the strength of the wound. Any intervention that increases the speed of this process (hematoma formation, migration and proliferation of fibroblasts, blood vessel formation, collagen production or remodeling process) can improve the wound healing process (3). A number of studies have shown that laser stimulation accelerates inflammation, regulates the level of prostaglandins, increases the activity of macrophages, increases the proliferation of fibroblasts and facilitates the synthesis of collagen.

    Laboratory studies have provided evidence to support the hypothesis of the effect of LLLT on the wound healing process. A number of researchers found that tissue stimulation with laser leads to the growth of capillaries, the formation of granulation tissue and changes in the production of cytokines. Increasing the number of cells (4), increasing DNA synthesis (5) and increasing collagen production (6) have been the claimed effects of LLLT on fibroblasts in vitro. Low power laser has been used in clinical interventions for more than 20 years and can regulate various biological processes (7).. Various lasers, including He-Ne, Nd:YAG, Ga-Al-As lasers have been used to obtain favorable clinical results in various treatments and programs, but the use of low-power lasers has not yet been widely accepted by the community of doctors and dentists. Perhaps the most important reason for this lack of acceptance is the lack of knowledge of doctors about the effects of LLLT at the cellular and molecular level (8). It is an ideal treatment because it removes the pocket and restores the tissue of the periodontium. Rapid recovery of the gums is also one of the important goals of perio specialists. The repair of damaged connective tissue after periodontal surgeries begins with the formation of bud tissue, and fibroblasts multiply in the bud tissue and produce collagen and other extracellular substrates (9). During the healing process, gingival fibroblasts secrete the extracellular matrix of the gingival connective tissue and are the origin of the formation of collagen fibers and their rapid and extensive remodeling (10).

    This study aims to investigate the effect of low-power Ga-Al-As laser on the proliferation and function of human periodontium fibroblasts (HFG3-P153 cell line) in vitro. .

    Abstract

    Introduction: Regeneratin of periodontium is the final goal of a perfect periodontal treatment. Proliferation of fibroblasts with regeneration of periodontal tissue is an ideal result of treatment. Use of low level laser is one of the suggested methods for increasing the fibroblast proliferation and improving the healing process. The aim of this study is to evaluate the effects of Ga-As laser on proliferation and activity of fibroblasts in an in vitro model.

    Material and method: Ga-Al-As laser with 810 nm wave length and power density of 50 nm was used in this study. Gingival fibroblast (HGI3-PI53) in test group were exposed to 4 J/CM2 LLLT in three consecutive days. 24, 48, 72 hours. after the exposure proliferation of fibroblasts were evaluated with the MTT test and the expression of collagen I gene were assessed through RT-PCR.

    Results: output of MTT test showed that in 0 and 24 hours after exposure there wasn't any significant difference between test and control group but the difference was significant after 48 and 72 hours. The result of RT-PCR test demonstrated that the expression of collagen I gene in test group was 4/7 times more than control group.

    Conclusion: application of Ga-As low-level laser ends to increased fibroblast proliferation rate and activity. Regarding the role of fibroblasts in periodontal regeneration and pocket elimination, the use of low-level laser in clinic can improve the efficacy of routine periodontal treatment.

  • Contents & References of Determining the effect of low power Ga-Al-As laser on the proliferation and function of human periodontal fibroblasts (cell line HFG3-P1 53) in an in vitro environment.

    List:

    Persian abstract. 1

    Introduction. 4

    Overview of texts. 7

    Tooth supporting tissues: 8

    Gum: 24

    Laser history 53

    Laser effects on tissues 60

    Laser in the treatment of chronic periodontitis 75

    Effective factors in periodontal regeneration process 80

    LANAP protocol 82

    Study objectives 89

    Materials and methods: 93

    Culturing of fibroblastic cells 93

    Low power laser irradiation 94

    Cell proliferation and growth test 95

    MTT method 95

    Vitality percent = (Vital cells / Control cells) × 100. 96

    Factors affecting MTT: 96

    Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) test 99

    Sample size: 102

    Results. 105

    Discussion: 110

    Suggestions. 116

    References. 119

    Abstract 130

    Source:

    References

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Determining the effect of low power Ga-Al-As laser on the proliferation and function of human periodontal fibroblasts (cell line HFG3-P1 53) in an in vitro environment.
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