The effect of citric, acetic and propionic acids spray on some microbial, chemical and appearance parameters of packaged chicken meat

Number of pages: 83 File Format: word File Code: 31958
Year: 2014 University Degree: Master's degree Category: Health - Health
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  • Summary of The effect of citric, acetic and propionic acids spray on some microbial, chemical and appearance parameters of packaged chicken meat

    Dissertation for Master's Degree in Food Health and Safety

    Abstract

    Context: Adding food acids to foods, in addition to inhibiting effects on microorganisms, creates the right taste and color in them.

    Purpose: The present study was conducted in order to determine the effect of organic acids on some microbial and organoleptic chemical indicators of chicken meat and increase its shelf life. Materials and methods: This study was conducted experimentally in Kohdasht city in 2013 on chicken meat. And to sample the meat of the chicken thighs after spraying with 1% sterile solutions of citric, acetic and propionic acids, packing and storing at 2-4 degrees Celsius and with 2-day intervals, it was tested and the significance of different treatments from the microbial point of view (counting of aerobic mesophiles, counting of coliforms, counting of thermophiles, counting of anaerobes), chemistry (PH and TVN) and Organoleptic (percentage of blood, quality of color and smell) was checked.                                                                                                   Findings: The results showed that the difference between the control sample and the sample treated with acetic and propionic acid was significant, and the difference in the aforementioned parameters between citric acid and acetic acid and propionic acid was significant (P < 0.01) and no difference was observed between acetic acid and propionic acid. According to the microbial, chemical and appearance parameters, the control sample can be treated for 4 days, treated with citric acid for 5 days, and acetic acid and Propionic acid was kept for 6-7 days. Conclusion: According to the results of this study, 1% concentration of these acids can be used to increase the shelf life of chicken meat.

    Key words: acetic acid, citric acid, propionic acid, shelf life, chicken meat rtl;">Introduction

    Based on the definition provided in the Codex[1] of food, the word "meat" is usually referred to all the edible parts of animals that are used to produce food needed by humans and includes meat and offal[2]. Meat is a collection of skeletal muscle tissues of livestock and slaughter birds, which is associated with fat, connective and bone tissues, lymph nodes and vessels (caplets, veins, capillaries, and lymphatic vessels) and related foundations, and makes up 45% of the weight of live animals and 70% of the weight of live birds. Microorganisms, and especially types of spoilage, are a very suitable environment for the activity of microorganisms, and if external factors are not controlled, they will spoil quickly. The shelf life of meat is significantly affected by the method of slaughtering and the quality of meat production and supply, because during these stages, the initial microbial load of meat is carried out in traditional slaughterhouses as a result of contamination with animal skin, intestinal contents, and water used in the slaughtering and production of animals in Iran. This means that after cutting the meat, it is packed in trays made of polystyrene and stretch film and after cooling, it is sold to the market. Due to the manipulation of meat during its cutting and packaging, the amount of meat contamination increases. Regardless of whether the weather conditions are hot or cold, packaging plays an important role in the production and transportation of meat and can be one of the useful things for optimizing meat production operations in developing countries. Packaging ensures that the meat is protected against loss of moisture, contamination with micro-organisms, discoloration and physical damage, and the color of the meat is more attractive to the consumer. Packaging of fresh meat can be done with a simple cover or by using advanced systems such as vacuum packaging and modified atmosphere packaging. The shelf life of meat pieces packed in a simple way at 4 degrees Celsius is 3 to 5 days. While using vacuum packaging and modified atmosphere packaging, the shelf life of fresh meat pieces or minced meat can be extended (Aalami et al, 2002; Rokni et al, 2001).                                Fresh meat can be done using a simple coating or using advanced systems such as vacuum packaging and modified atmosphere packaging. The shelf life of meat pieces packed in a simple way at a temperature of 4 degrees Celsius is 3 to 5 days. While using vacuum packaging and modified atmosphere packaging, the shelf life of fresh meat pieces or minced meat can be extended (Rokni, 1998; Jey, 2005; Davidson et al, 2005). The natural pH level of meat may also prevent the growth of some bacteria, but it promotes the growth of another group of microorganisms (Razavilar, 1998). Low pH in the range of 4 to 4.5 prevents the growth of spoilage microorganisms and pathogenic microorganisms.  To reduce the pH of food, it is possible to use organic acids such as citric acid, benzoic acid, acetic acid, lactic acid, propionic acid, etc., which are considered edible acids (Ghasemian, 2000). RTL; "> Effect of steak, citric and propionic acid spray on some microbial, chemical and appearance parameters of packed poultry meat

    1-2-2- 2-2-2 Aerobic Count Counts Counting of anaerobes Measuring PH and TVN Appearance characteristics of packaged chicken meat under the effect of organic acids in Kohdasht city in 1992 Increasing the shelf life of packaged chicken meat is under the effect of organic acids.

    1-3- Assumptions or research questions

    Using propionic acid, citric acid and acetic acid, the shelf life of meat at refrigerator temperature is longer.                                                                               The use of propionic, citric and acetic organic acids does not have an adverse effect on the smell and appearance of meat

    Abstract

    Background: Adding edible acids to foodstuffs not only makes a control in microorganisms, but also causes an appropriate taste and color.

    Objective: The main aim of this study was the relationship between the effect of organic acids on microbial, chemical and organoleptic and its shelf life chicken's meat.

    Methods: This experimental study was conducted on chicken's meat. At first chicken's meat with 1% solution of sterilized citric, acetic, and propionic acids, and then packed. The samples of packed meats were kept at temperature below 4 degrees Celsius, and at intervals of 2 days tested durations. Different statistical analyzes of parameters were investigated through microbial count of aerobic mesophylls, coliforms, psychotropics and nonaerobics), chemical (PH, Total volatile nitrogen, TVN) and organoleptic (percentage of plasma, smell and taste quality).

    Findings: The results showed that the difference between control and treatment samples with acetic acid and propionic was significant (P<0.01). Also, the difference between control and treatment of acetic acid, propionic acid and citric acid was significant (P<0.01). The difference between acetic acid and propionic acid was not significant. By regarding the microbial and chemical parameters, we can preserve the control for 4 days, treatment with citric acid for 5 days and care samples with acetic and propionic acids until 6-7 days.

  • Contents & References of The effect of citric, acetic and propionic acids spray on some microbial, chemical and appearance parameters of packaged chicken meat

    List:

     

    The first chapter. 1

    Introduction and statement of the problem. 1

    1-1- Introduction. 2

    1-2- Objectives. 3

    1-2-1- Main goals. 3

    1-2-2- sub-goals. . 3

    1-2-3- practical purpose. 4

    1-3- Assumptions or research questions. 4

    The second chapter. 5

    Text review. 5

    2-1-meat. 6

    2-2-quality and physical sensory characteristics of meat. 7

    2-2-3- freezing point. 8

    2-2-4-PH. 9

    2-2-5-blood seepage. 9

    2-2-6-crating (caging) and transportation. 10

    2-3- Quality and chemical characteristics. 12

    2-3-1- Water. 13

    2-3-2 protein. 13

    2-3-3- Leads. 13

    2-3-4- sugars (carbohydrates) 14

    2-3-5- vitamins. 14

    2-3-6- Minerals. 14

    2-3-7 - Volatile nitrogen) (TVN. 14

    2-4- Quality and characteristics of microbiology. 15

    2-4-1- Microbial flora of fresh meat. 15

    2-2-4- Microbial flora of fresh chicken. 16

    2-5- Bacteriological standards and criteria. 16

    2-6- Microbial foodborne diseases caused by consumption of contaminated meat. 18

    2-5-1- Salmonella

    2-5-2- Campylobacter jejuni 19

    2-5-3- Escherichia coli 20

    2-5-4- Listeria monocytogenes 20

    2-5-5- Yersinia enterocolitica 20

    2-5-6- Closteridium perfringenes 21

    2-5-7- Staphylococcus aureus 21

    2-5-7- Bacillus cereus

    2-6- Sources of contamination of meat and meat products. 22

    2-6- Workers. 23

    2-6-4 Equipment and tools 23-6-5 slaughtering process 2-7- Reduction of meat quality. 25

    2-7-1- Loss of appearance quality. 25

    2-7-2- Chemical and biochemical changes. 26

    2-7-2-1 Natural changes after killing. 26

    2-7-2-2- Chemical changes during meat storage (chemical spoilage) 28

    2-8- Microbiological changes (microbial spoilage). 30

    2-8-1- General types of microbial spoilage. 30

    2-8-1-1- Corruption in aerobic conditions. 30

    2-8-1-2- Corruption in anaerobic conditions. 32

    2-8-2- Microbial spoilage of all types of meat. 34

    2-8-2-1- Fresh meat (without packaging) 34

    2-8-2-2- Packaged meat stored in the refrigerator. 35

    2-8-2-3- Vacuum packed meat. 36

    2-8-2-4- meat packed with modified atmosphere. 37

    2-9- Packaging. 37

    2-10- Removing microorganisms. 39

    2-10-1- carcass modification. 39

    2-10-2- Chemical sanitization of the carcass. 39

    2-2-3- Use of cold. 40

    2-10-4-freezing. 42

    2-11- Shelf life of meat and its products. 42

    2-11-1-red meat. 42

    2-11-2- Frozen meat. 45

    2-11-3- fresh/frozen chicken meat. 45

    The third chapter. 47

    Research method. 47

    3-1- Type of study. 48

    3-2- Implementation method and research design. 48

    3-2-1- Sampling. 48

    3-2-2-General enumeration of aerobic mesophilic bacteria. 49

    3-2- 3- Counting the cold ones. 52

    3-2-4- Measurement of Volatile Nitrogen (TVN)) Total Voletila Nitrogen) 53

    3-2-5- Determination of PH of meat. 54

    3-2-6- Statistical analysis. 56

    3-2-7- Test place. 56

    Chapter Four. 57

    Findings. 57

    4-1- Aerobic mesophilic bacteria. 58

    4-2- Anaerobic bacteria. 58

    4-3- coliform bacteria. 59

    4-4- Cryogenic bacteria. 59

    4-5- Total nitrogen escape. 60

    4-6- PH. 61

    4-7- Blood. 61

    4-1- Color. 62

    4-2- smell. 63

    The fifth chapter. 64

    Discussion and conclusion. 64

    5-1 - Discussion and conclusion. 65

    5-2- Suggestions. 68

    List of references 69

    Source:

     

     

    Aalami, M. and Khamesian, A. (2002). The microbiology of meat and poultry. Jahad Research and Education publication, pp. 330-331 and 368-384.

    Aalami, M.and Khamesian, A. (2000). The microbiology of meat and poultry. Jahad Research and Education publication, pp:128.

     

    Adams, M. R. Moss. M. O. (2002). Food Microbiology, The Royal Society of Chemistry; pp:157-159

     

    Anonymous, AOAC. (1995). Official Methods of Analysis AOAC International. 16th edition, Vol: 2, chap. 39, pp. 5-6.

     

    Berry, E. D. and Cutter, C. N. (2000). Effect of acid adaptation of Escherichia coli O157:H7 on efficacy of acetic acid spray washes to decontaminate. Applied and Environmental Microbiol ogy, 66: 1493-1498.

    Bremner, A, Johnston, M . (1996). Poultry meat hygiene and inspection, First edition, W. B Sauders Publishing co. pp: 217-219

    Carrick, E. D., D. J. C. Wild, L. Tracey, R. Harvey, 2001. Factors affecting tenderness of electrically stimulated poultry. New Zealand J. Agric. Research. 44: 171-175.

    Castillo, A., Lucia, L. M., Goodson, K. J., Savell, J. W. and Acuff, G. R. (1998). Comparison of water wash, trimming and combined hot water and lactic acid treatments for reducing bacteria of fecal origin on beef.                                                               Codjoe, K. S. (1998). The effect of lactic acid sprays on the keeping qualities of meat during storage. International Journal of Microbiology, 7:1-7.

    Davidson, P. M., Sofos, J. N. and Branen, A. L. (2005). Antimicrobials in food, 3rd edition, CRC Press, pp. 94-99 and 107-110.                                                                                                        

    Davies, A. Board, R. (2002).  The microbiology of meat poultry, p: 214.

    Debut, M., C. Berri, E. Baeza, N. Sellier, C. Arnould, D. Guemene, N. Jehl, B. Boutten, Y. Jego, C. Beaumont, and E. Le Bihan-Duval, 2003. Variation of chicken technological meat quality in relation to genotype and preslaughter stress condition. J. Poult Sci. 82: 1829-1838.

    Downes, F. P. and Ito, K. (2001). Compendium of methods for the microbial examination of foods, 4th edition, American public health association, pp. 63-67, 69-76 and 159-164.   Eero, J., Puolanne, A. and Poso, R. (2003). Lactic acid in muscle and its effects on meat quality, American Meat Science Association, pp. 55-62.

    Eleftherios, H., Drosinos, M. M., Aikaterini, K., Dimitrios, K. and Ioannis, M. (2006). Inhibitory effect of organic acid salts on spoilage flora in culture medium and cured cooked meat products under commercial manufacturing conditions. Meat Science, 73: 75-81.

    Ekstrand, C. 1998. An observational cohort study of the effects of catching method on carcass rejection rates in broilers (Abstract). Anim. Welf. 7: 87-96

    Eric, P. B., Effect of stress on meat yield and quality. University of Missouri, MO 65211.

    . Fatemi, H. (2001). Food chemistry. Sherkat Sahami Enteshar, pp. 420-423 and 429-431.                   Ghasemian, S. H. (2000). food microbiology. 1st edition, Amani Publication, pp. 183.

    Fazara. A. Ghaem maghami. S. (2002). quality control food of animal products. Jahad Research and Education publication, pp:124-125. 

    Glian.A. Moeini. M. (1994). Nutrition poultry.  Unit education Publication, pp: 23           Groom,.G. M., 1990. Factors affecting poultry meat quality. UDAS. Ministry of Agriculture, England.

    Hanifian, Sh. (2008). Control of chilled beef spoilage by combination of packaging and organic acid treatments. Journal of specialized veterinary medicine, Islamic Azad university of Tabriz, 1: 177-185. 

    Institute of standard and industrial research of Iran (1990). Total bacterial count. No. 356. Institute of standard and industrial research of Iran (1984). Acceptable microbial load in meat. No. 2394. Jay, J. M. (2005).

The effect of citric, acetic and propionic acids spray on some microbial, chemical and appearance parameters of packaged chicken meat