Contents & References of Comparison of kinetic characteristics of free and immobilized lipase enzyme on chitosan nanoparticles
List:
Abstract: . 1
Chapter One: Preliminaries and Generalities
1-1- Statement of the problem. 3
1-2-Research objectives. 4
1-3-Research hypotheses. 4
1-4- The concept of nanotechnology. 5
1-4-1-Nanotechnology applications. 5
1-4-1-1- Production of industrial materials and products. 5
1-4-1-2- Medicine and the human body. 6
1-4-1-3- Sustainability of resources. 6
1-4-1-4- food industry. 6
1-5-enzymes. 8
1-5-1-Properties of enzymes. 8
1-5-2- Characteristics of enzymatic reactions. 11
1-5-3- parameters affecting enzyme reactions. 14
1-5-3-1- Temperature. 14
1-5-3-2-pH. 14
1-5-3-3-inhibitors. 15
1-5-4- Enzyme reaction kinetics. 16
1-5-5-factors affecting the speed and activity of enzymes. 17
1-5-5-1-Effect of raw material concentration on enzyme reaction speed. 17
1-5-6- Michaelis-Menten equation. 18
1-5-7- Lenoir-Burke equation. 21
1-5-8-lipase enzyme. 23
1-5-8-1-use of lipase. 25
1-5-8-2-lipase reaction kinetics. 27
1-5-8-3-Methods for measuring lipase activity. 28
1-6- enzyme stabilization. 30
1-6-1- Choosing the right base and method to stabilize the enzyme. 31
1-7- Chitin and chitosan. 32
1-7-1- Chitin. 33
1-7-2- chitosan. 34
1-7-2-1- Applications of chitosan. 35
1-8-Determining the characteristics of chitosan nanoparticles. 38
Chapter Two: Literature and Research Background
2-1-Review of enzyme studies. 41
2-2-Review on the background of lipase enzyme. 42
2-3-History of chitin and chitosan production. 44
2-4-History of enzyme immobilization on nanoparticles. 45
2-5-Previous research in this field. 46
Chapter Three: Materials and Methods
3-1- Introduction. 51
3-2-Chemicals. 52
3-3-Required means and devices. 54
3-3-1- Laboratory containers. 54
3-3-2-devices. 54
3-4-Laboratory methods used in the thesis. 58
3-4-1-synthesis of chitosan nanoparticles. 58
3-4-2-Determination of lipase enzyme by covalent binding method based on Schiff base reaction 59
3-4-3-Determining the properties of chitosan nanoparticles using scanning electron microscope 60
3-4-4-Determining the size distribution of synthesized nanochitosan particles by light diffraction method using Zetasizer 61
3-4-5-infrared spectroscopy (FT-IR). 62
3-4-6- Total protein measurement by Bradford method. 62
3-4-7-Measuring the amount of protein (lipase) immobilized on chitosan nanoparticles. 63
3-4-8-measurement of lipase enzyme activity. 63
3-4-8-1-standard curve of lipase activity. 64
3-4-8-2-first method- measurement of lipase activity using the Pars test kit (Lipase DC quantitative diagnostic kit) in serum or plasma by photometric method. 66
3-4-8-3-second method- measurement of lipase activity using the substrate paranitrophenyl palmitate (pNPP). 68
3-4-9- Examining the effect of temperature on the activity of lipase enzyme in the free state and immobilized on nano chitosan 68
3-4-10-Investigating the effect of pH on the activity of lipase enzyme in the free state and immobilized on nano chitosan 69
3-4-11-Investigating the kinetic parameters of lipase enzyme free and immobilized on chitosan nanoparticles 69
Chapter Four: Results
4-1-Introduction. 71
4-2-Scanning electron microscope (SEM) imaging. 71
4-3-Fourier transform infrared spectroscopy (FT-IR). 72
4-4- Size distribution of nanoparticles synthesized using zeta sizer. 74
4-5-Pseudomonas lipase activity free and stabilized on chitosan nanoparticle. 75
4-6-Investigating the effect of pH on the activity of free and immobilized Pseudomonas lipase enzyme on chitosan nanoparticle 76
4-7-Comparison of the relative activity percentage of free and immobilized lipase enzyme at different pH 77
4-8-Investigating the effect of temperature on the activity of free and immobilized Pseudomonas lipase enzyme on chitosan nanoparticle. 78
9-4-Comparison of the percentage of relative activity of free and stabilized lipase enzyme at different temperatures 79
4-10-Kinetic parameters of free and stabilized Pseudomonas lipase enzyme on chitosan nanoparticles 80
Chapter Five: Discussion. 78
9-4-Comparison of the relative activity percentage of free and stabilized lipase enzyme at different temperatures 79
4-10-Kinetic parameters of free and stabilized lipase pseudomonas enzyme on chitosan nanoparticle 80
Chapter five: discussion and conclusion
Discussion. 83
Conclusion. 87
Problems and suggestions. 89
Sources and sources. 90
List of Persian sources. 91
List of non-Persian sources. 92
English abstract. 100
Source:
]1[ Alamzadeh; Iran, 1377, enzyme processes, Sharif University of Technology Publishing Institute. Manouchehr, 1380, Biochemical Engineering, Sharif University of Technology Publishing Institute.
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[4] Mubarak Qamsari, Elaha, Kasri Kermanshahi, Ruha, and Mousavinejad, Zahra. 1389, investigating the effect of pH on the stability and activity of Pseudomonas aeruginosa lipase isolated from oily wastewater, the fourth specialized conference on environmental engineering, Tehran University, Faculty of Environment. pp. 1-5. [5, 5, 20, Dostgani, Amir, Vashghani Farahani, Ebrahim, Imani, Mohammad, 1386, determining the optimal conditions for preparing nanoparticles from the natural polymer chitosan, Journal of Polymer Science and Technology, 8th International Seminar on Polymer Science and Technology, pp. 464-457.
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